Basic Techniques in Biochemistry and Molecular Biology

R.K. Sharma, S.P.S. Sangha

ISBN: 9789389583694

INR 345


This book includes 15 chapters covering more than 135 experimental protocols. It discusses all the relevant topics like pH and buffers, spectrophotometry, chromatography, carbohydrates, lipids, proteins, electrophoresis, enzyme immunology, vitamins and pigments, metabolites and molecular biology. It includes a wide range of experiments from preparation of culture media to PCR, Southern and Western blotting. All the experiments have been meticulously designed and special care has been taken to the safety in laboratory and precautions are given wheresoever required.


  • General Introduction
  • pH and Buffers
  • Spectrophotometry
  • Centrifugation and Cell Fractionation
  • Chromatography
  • Carbohydrates
  • Lipids
  • Amino Acids and Proteins
  • Nucleic Acids
  • Electrophoresis
  • Immunology
  • Enzymes
  • Metabolites
  • Vitamins, Hormones and Pigments
  • Molecular Biology
  • Appendices



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