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Essentials of Biotechnology

Ulhas K. Patil, Kalyani Muskan

ISBN: 9789389633313

INR 495


Essentials of Biotechnology is meant for undergraduate biotechnology and life sciences’ students. The book discusses the basics of interdisciplinary subjects which is required for developing the conceptual understanding in biotechnology and to acquire research attitude. It elaborates fundamental concepts which are absolutely necessary for budding biotechnologists. It is an attempt to cover broad spectrum of biological dimensions with biotechnological exploration.



  1. Introduction to Biotechnology
  2. Cytology  
  3. Growth
  4. Nutrition
  5. Carbohydrates
  6. Lipids
  7. Amino Acids and Proteins
  8. Nucleic Acids  
  9. Vitamins
  10. Enzyme
  11. Hormones and Phytohormones
  12. Biophysical Chemistry  
  13. Microscopy
  14. Staining
  15. Cell Measurements
  16. Isolation and Cultivation Techniques
  17. Sterilization and Disinfection
  18. Methods in Microbial Taxonomy



  1. Safety in Laboratory
  2. Preparation of Standard Solutions
  3. Specific Gravity Determination
  4. Viscosity Measurement
  5. pH Meter
  6. Beer’s and Lambert’s Law
  7. Paper Chromatography
  8. Thin Layer Chromatography
  9. Qualitative Tests for Carbohydrates
  10. Qualitative Tests for Proteins
  11. Amino Acids
  12. Determination of pKa Value
  13. Isolation of Starch from Potato
  14. Isolation of Casein from Milk
  15. Estimation of Protein (Biuret Assay)
  16. Estimation of Sugar (DNSA Method)
  17. Determination of Iodine Number
  18. Estimation of Acid Number
  19. Estimation of DNA (DPA Method)
  20. Estimation of RNA  (Orcinol  Method)
  21. Enzyme Assay (Amylase)
  22. Compound Microscope
  23. Culture Media Preparation
  24. Negative Staining (Relief Staining)
  25. Monochrome  Staining
  26. Gram Staining
  27. Streak Plate Technique
  28. Pour Plate Technique
  29. Most Probable Number (MPN)
  30. Separation of Serum from Blood
  31. Blood Group
  32. Antigen-Antibody Reaction
  33. Widal Test
  34. Isolation of Genomic DNA
  35. Isolation of Plasmid DNA
  36. SDS-Page
  37. Agarose Gel Electrophoresis

References / Index