Essentials of Biotechnology
ISBN: 9789389633313
Description
Essentials of Biotechnology is meant for undergraduate biotechnology and life sciences’ students. The book discusses the basics of interdisciplinary subjects which is required for developing the conceptual understanding in biotechnology and to acquire research attitude. It elaborates fundamental concepts which are absolutely necessary for budding biotechnologists. It is an attempt to cover broad spectrum of biological dimensions with biotechnological exploration.
Units
- Introduction to Biotechnology
- Cytology
- Growth
- Nutrition
- Carbohydrates
- Lipids
- Amino Acids and Proteins
- Nucleic Acids
- Vitamins
- Enzyme
- Hormones and Phytohormones
- Biophysical Chemistry
- Microscopy
- Staining
- Cell Measurements
- Isolation and Cultivation Techniques
- Sterilization and Disinfection
- Methods in Microbial Taxonomy
Experiments
- Safety in Laboratory
- Preparation of Standard Solutions
- Specific Gravity Determination
- Viscosity Measurement
- pH Meter
- Beer’s and Lambert’s Law
- Paper Chromatography
- Thin Layer Chromatography
- Qualitative Tests for Carbohydrates
- Qualitative Tests for Proteins
- Amino Acids
- Determination of pKa Value
- Isolation of Starch from Potato
- Isolation of Casein from Milk
- Estimation of Protein (Biuret Assay)
- Estimation of Sugar (DNSA Method)
- Determination of Iodine Number
- Estimation of Acid Number
- Estimation of DNA (DPA Method)
- Estimation of RNA (Orcinol Method)
- Enzyme Assay (Amylase)
- Compound Microscope
- Culture Media Preparation
- Negative Staining (Relief Staining)
- Monochrome Staining
- Gram Staining
- Streak Plate Technique
- Pour Plate Technique
- Most Probable Number (MPN)
- Separation of Serum from Blood
- Blood Group
- Antigen-Antibody Reaction
- Widal Test
- Isolation of Genomic DNA
- Isolation of Plasmid DNA
- SDS-Page
- Agarose Gel Electrophoresis
References / Index