Plant Tissue Culture: Practices and New Experimental Protocols

B.N. Sathyanarayana, Dalia B. Varghese

ISBN: 9789390581207

318 pages


INR 1595


Plant Tissue Culture forms an integral basis of the present day biotechnology. Plant Tissue Culture: Practices and New Experimental Protocols is being brought out to fill the existing gap in the available literature on plant tissue culture, especially focusing on the aspects of practical procedures and protocols of tissue culture. This book contains important experimental techniques and gives guidance on carrying out hands-on experiences. It has been designed in a simple way, giving all the necessary procedures as a general guideline and also necessary tips to maneuver any problem encountered.

  • Historical Developments in Plant Tissue and Cell Culture
  • Laboratory Design and Development
  • Instrumentation
  • Plant Tissue Culture Media
  • Sterilization Techniques
  • Safety Rules and Regulations
  • Applications of Plant Tissue Culture
  • Micropropagation
  • Micropropagation by Proliferation of Auxillary Buds
  • Micropropagation by Adventitious Shoot Regeneration
  • Plant Regeneration from Callus Cultures
  • Plant Regeneration by Somatic Embryogenesis
  • Meristem Culture
  • In vitro Haploid Production
  • Embryo Culture
  • Endosperm Culture
  • Culturing of Roots in vitro
  • Protoplast Isolation and Culture
  • Protoplast Fusion and Cybridization
  • Storage Organ Culture
  • In vitro Germplasm Conservation
  • In vitro Production of Plant Secondary Metabolites
  • Acclimatization of Micropropagated Plantlets
  • In vitro and ex vitro Rooting of Micro Shoots
  • Somaclonal and Gametoclonal Variations
  • Agrobacterium Mediated Transformation
  • Problems in Plant Tissue Culture
  • Commercial Micropropagation
  • Microscopic and Photographic Techniques for Plant Tissue Culture
  • Histological Techniques
  • Glossary
  • Appendix
  • Selected Bibliography Readership: Graduate, Postgraduate and Research Students of Plant Tissue Culture, Biotechnology, Botany, Microbiology, Biochemistry, Agriculture, Horticulture and Allied Science


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