Gene Cloning and DNA Analysis, 8ed: An Introduction(An Indian Adaptation)
ISBN: 9789354643644
472 pages
For more information write to us at: acadmktg@wiley.com
Description
Gene Cloning and DNA Analysis: An Introduction, Eighth Edition is a comprehensive and authoritative textbook that explores all of the topics crucial to an understanding of gene cloning in an approachable way. This Adapted Edition has been developed based on the detailed feedback obtained from long-standing users of the book about how the book should be revised. Building on the strength of the previous editions, the focus of this adaptation has been on strengthening the concept coverage.
Part I The Basic Principles of Gene Cloning and DNA Analysis
1 Why Gene Cloning and DNA Analysis are Important
2 Vectors for Gene Cloning: Plasmids and Bacteriophages
3 Isolation and Purification of DNA from Living Cells
4 Manipulation of Purified DNA
5 DNA Amplification and Analysis Using Polymerase Chain Reaction
6 Introduction of DNA into Living Cells
7 Cloning Vectors for Prokaryotes (E. coli)
8 Cloning Vectors for Eukaryotes
9 How to Obtain a Clone of a Specific Gene
Part II The Applications of Gene Cloning and DNA Analysis in Research
10 Sequencing Genes and Genomes
11 Studying Gene Expression and Function
12 Studying Genomes
13 Studying Transcriptomes and Proteomes
Part III The Applications of Gene Cloning and DNA Analysis in Biotechnology
14 Production of Protein from Cloned Genes
15 Use of Recombinant DNA Technology in Medicine
16 Use of Recombinant DNA Technology in Agriculture
17 DNA Analysis in Forensic Science and Archaeology
Appendix A: Vectors Used for Transformation
Appendix B: Genetically Modified Organisms
Glossary
Index